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- W2392743043 abstract "Objective To detect suspected field samples of plague by PCR to establish accurate,simple and rapid diagnostic technique.Methods DNA of dead rodents found in epidemic focus was extracted by two ways to detect caf1,pla and several tag sequences in chromosome.Results Caf1,pla(their homology with Yersinia pestis caf1,pla from GenBank was more than 99%) and four tag sequences in chromosome ypo 2087,ypo 2090,ypo 2094 and ypo 2102 were found from the two samples in gene amplification,and sensitivity was higher used by DNA extract kits than by boiling.Conclusions Positive rate of diagnosis of plague by PCR could be increased by optimization of templet collection,improvement of amplification efficiency,and multiple genes test in both plasmids and chromosome." @default.
- W2392743043 created "2016-06-24" @default.
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- W2392743043 date "2013-01-01" @default.
- W2392743043 modified "2023-09-27" @default.
- W2392743043 title "Study on application of PCR to detect field samples of plague" @default.
- W2392743043 hasPublicationYear "2013" @default.
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