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- W2392751613 abstract "Purpose To study the apoptosis of the Croton alkaloids(CA)in Hela cervical cancer cells in vitro and its mechanism.Methods Inhibition of the cell proliferation was measured by MTT assay.Cell apoptosis was detected by Annexin V-PI double staining.The Caspase-8 activity was detected by Caspase-8 Activity Assay Kit and the expressions of caspase-8 mRNA was tested by RT-PCR.Results CA in hibit-ed the proliferation of Hela cells in a dose-dependent manner.FMC could detect the cell apoptosis.FMC results suggested that CA could induce apoptosis in Hela cells.Caspase-8 activity was up-regulated obviously and the level of Caspase-8 mRNA was increased after CA treatment.Conclusion CA displays a proliferation inhibitory apoptosis-inducing effect in Hela cells.The molecular mechanism of apoptosis Hela cells induced by CA has possibly related to up-regulating expression of caspase-8 gene." @default.
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- W2392751613 date "2010-01-01" @default.
- W2392751613 modified "2023-09-23" @default.
- W2392751613 title "The apoptosis of Hela cells induced by croton alkaloid and its mechanism" @default.
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