Matches in SemOpenAlex for { <https://semopenalex.org/work/W2392752863> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2392752863 abstract "Objective To construct eukarytic co-expression vector for rat endostatin cDNA and anti-sense /sense-VEGF164 cDNA and to transfect it into C6 glioma cells. Methods cDNA encoding rat endostatin and cDNA encoding anti-sense /sense-VEGF164 were amplified from newborn rat brain tissue with RT-PCR and inserted into the eukarytic vector pBudCE 4.1 sequently, recombinants were identified by restriction enzymes digestion, PCR and the nucleotide sequencing of the target gene. After successful reconstruction of the genes of endostatin and anti-sense /sense-VEGF164, the recombinants were transfected into C6 cells by lipofectin technique. The positive clones were screened out through zeocin resistance selection. The endostatin in supernate from the positive clones was identified by Western-blot and MTT method. With immunocytochemistry, the endostatin in the positive clones were located. The quantity of VEGF in supernate from the positive clones was quantitated by ELISA assay. Results The sizes of the amplified endostatin gene fragment and VEGF gene fragment were in accordance with that we expected. And the gene sequence inserted into the eukarytic vector pBudCE 4.1, were consistent with the known sequence. The endostatin with biological activities can be secreted from the clones transfected with endostatin cDNA, and the down-regulation of VEGF expression was found in the clones transfected with recombinants of endostatin cDNA or /and anti-sense-VEGF164 cDNA. Conclusion The recombinant rat endostatin gene clone has been established and cloned into the eukarytic vector pBudCE 4.1 successfully, the endostatin can expressed in C6 cells; the expression of VEGF in C6 glioma cell can be down-regulated by endostatin." @default.
- W2392752863 created "2016-06-24" @default.
- W2392752863 creator A5084330155 @default.
- W2392752863 date "2005-01-01" @default.
- W2392752863 modified "2023-09-23" @default.
- W2392752863 title "Construction of eukarytic co-expression vector for rat endostatin cDNA and anti-sense /sense-VEGF164 cDNA and its expression in C_6 glioma cells" @default.
- W2392752863 hasPublicationYear "2005" @default.
- W2392752863 type Work @default.
- W2392752863 sameAs 2392752863 @default.
- W2392752863 citedByCount "0" @default.
- W2392752863 crossrefType "journal-article" @default.
- W2392752863 hasAuthorship W2392752863A5084330155 @default.
- W2392752863 hasConcept C104317684 @default.
- W2392752863 hasConcept C153911025 @default.
- W2392752863 hasConcept C187882448 @default.
- W2392752863 hasConcept C2780394083 @default.
- W2392752863 hasConcept C33161422 @default.
- W2392752863 hasConcept C40767141 @default.
- W2392752863 hasConcept C54009773 @default.
- W2392752863 hasConcept C54355233 @default.
- W2392752863 hasConcept C75920679 @default.
- W2392752863 hasConcept C81089528 @default.
- W2392752863 hasConcept C86803240 @default.
- W2392752863 hasConceptScore W2392752863C104317684 @default.
- W2392752863 hasConceptScore W2392752863C153911025 @default.
- W2392752863 hasConceptScore W2392752863C187882448 @default.
- W2392752863 hasConceptScore W2392752863C2780394083 @default.
- W2392752863 hasConceptScore W2392752863C33161422 @default.
- W2392752863 hasConceptScore W2392752863C40767141 @default.
- W2392752863 hasConceptScore W2392752863C54009773 @default.
- W2392752863 hasConceptScore W2392752863C54355233 @default.
- W2392752863 hasConceptScore W2392752863C75920679 @default.
- W2392752863 hasConceptScore W2392752863C81089528 @default.
- W2392752863 hasConceptScore W2392752863C86803240 @default.
- W2392752863 hasLocation W23927528631 @default.
- W2392752863 hasOpenAccess W2392752863 @default.
- W2392752863 hasPrimaryLocation W23927528631 @default.
- W2392752863 hasRelatedWork W2348380955 @default.
- W2392752863 hasRelatedWork W2351763675 @default.
- W2392752863 hasRelatedWork W2351811759 @default.
- W2392752863 hasRelatedWork W2352252022 @default.
- W2392752863 hasRelatedWork W2354139735 @default.
- W2392752863 hasRelatedWork W2354786885 @default.
- W2392752863 hasRelatedWork W2355098103 @default.
- W2392752863 hasRelatedWork W2356061815 @default.
- W2392752863 hasRelatedWork W2356886803 @default.
- W2392752863 hasRelatedWork W2359070436 @default.
- W2392752863 hasRelatedWork W2359657466 @default.
- W2392752863 hasRelatedWork W2372154035 @default.
- W2392752863 hasRelatedWork W2375771671 @default.
- W2392752863 hasRelatedWork W2376840534 @default.
- W2392752863 hasRelatedWork W2377590043 @default.
- W2392752863 hasRelatedWork W2380761158 @default.
- W2392752863 hasRelatedWork W2382819712 @default.
- W2392752863 hasRelatedWork W2384026624 @default.
- W2392752863 hasRelatedWork W3147821787 @default.
- W2392752863 hasRelatedWork W2852675405 @default.
- W2392752863 isParatext "false" @default.
- W2392752863 isRetracted "false" @default.
- W2392752863 magId "2392752863" @default.
- W2392752863 workType "article" @default.