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- W2392761486 abstract "Objective:To develop a model of orthotopic bladder cancer model of nude mice using a new reproducible technique. Methods: Thirty-six nude mice were devided into three groups:group A,B and C.In group A,2×106 T24 cells were instilled into bladders whose mucosa had been scuffed by a needle and that had been instillated with Poly-L-Lysine previously. In group B,2×106 T24 cells were instilled into bladders whose mucosa had only been scuffed by a needle. In group C,2×106 T24 cells were instilled into bladders which had been instillated with Poly-L-Lysine previously.In 90 days,mice were carefully monitored for health status and hamaturia.Nude mice were euthanatized if their body weigth loss were greater than 10% or a dyscrasia were noticed. Results: In group A,all 12 nude mice reached their end points within 70 days,the tumor forming rate is 100% by pathology.In group B,9 mice reached their end points and the others survived within 90 days,the tumor forming rate is 75%,In group C,all 12 nude mices survived within 90 days and tumor forming rate is zero. Conclusion: The establishment of a orthotopic bladder cancer model of mude mice by scuffing mucosa and instillating Poly-L-Lysine is a stable and reproducible method." @default.
- W2392761486 created "2016-06-24" @default.
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- W2392761486 date "2009-01-01" @default.
- W2392761486 modified "2023-09-23" @default.
- W2392761486 title "A New Method of Establishing an Orthotopic Murine Bladder Cancer Model" @default.
- W2392761486 hasPublicationYear "2009" @default.
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