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- W2392764281 abstract "Amplified fragment length polymorphism(AFLP) is an efficient technique to identify DNA fragment length polymorphism,which was widely used for genetic diversity analysis,construction of genetic maps and genes mapping in fungi,animals,plants and insect.But some works could not get expectant result while amplified fragment length polymorphism included sequences polymorphism,single nucleotide polymorphisms(SNPs) and insertions and deletions(indel).To understand DNA sequence polymorphism of wheat AFLP fragments,we compared 30 pair fragments in which every pair fragments were the same size but from different cultivars,and other 15 pair fragments in which every pair fragments from the same band.The sequences of every pair fragments were not all identical.The difference of each pair fragments were 1%～70%.Even non-polymorphic bands between cultivars contained multiple fragments with sequence difference.In this study we present sequence differences of 95 pair fragments with the same size from 64 wheat AFLP bands and they should locate different loci or alleles among chromosome A,B and D.That is the reason of some AFLP markers could not be mapped and some wheat varieties could not be clustered in the right group by AFLP markers.This paper also describes the method for the conversion of AFLP markers to sequence characterized amplified region(SCAR) markers based on sequence polymorphisms of AFLP fragments for single locus and SNP assays." @default.
- W2392764281 created "2016-06-24" @default.
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- W2392764281 date "2007-01-01" @default.
- W2392764281 modified "2023-09-26" @default.
- W2392764281 title "Sequence Polymorphism of Wheat AFLP Fragments and Conversion of AFLP Marker to SCAR Marker" @default.
- W2392764281 hasPublicationYear "2007" @default.
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