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- W2392783234 abstract "Objective To investigate a valuable method of gene therapy to treat chronic arterial ischemic diseases in lower extremity. Methods VEGF was selected as the target therapeutical gene. Recombinant plasmid phVEGF 165 , constructed in vitro as the eukaryotic vector of human VEGF cDNA, was injected into the ischemic hindlimb muscles of rabbit models. In situ hybridization and RT-PCR were carried out to detect the expression of hVEGF 165 in the muscle. Immunohistochemistry assay and Western blotting were carried out to detect the expression of hVEGF 165 protein. Results The expression of external hVEGF 165 peaked at 1-week post-injection and sustained 2 ～ 3 weeks. The expression of protein peaked at 2-weeks post-injection and lasted 4 week. Conclusion Skeletal muscle could extract and express external hVEGF 165 , and further translate the external gene into protein. The effects of direct intramuscular injection of phVEGF 165 were limited in local muscles, but no longer than 1 month. This method of gene therapy is quite safe." @default.
- W2392783234 created "2016-06-24" @default.
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- W2392783234 date "2002-01-01" @default.
- W2392783234 modified "2023-09-25" @default.
- W2392783234 title "Detection of the Expression of hVEGF and Its Protein after in situ Intramuscular Transfection of phVEGF_(165)" @default.
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