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- W2392792600 abstract "Objective To compare the detection results by multiplex realtime reverse transcription-polymerase chain reaction(RT-PCR)and tradional RT-PCR in hand,foot and mouth disease(HFMD).Methods 86 throat swab specimens of mild cases and 120 throat swab specimens of serious cases were detected by multiplex realtime RT-PCR and traditional RT-PCR.The detection results of two assays were compared and analysed.Results The multiplex realtime RT-PCR had much higher sensitivity than the traditional RT-PCR for detection of enterovirus with throat swab specimens.The positive detection rate of enterovirus in mild HFMD cases was raised from 52.3% to 93.0%,in severe cases was raised from 8.3% to 60.0% by realtime RT-PCR.And the propotion of enterovirus type 71,coxsackievirus group A type 16 and other enterovirus in HFMD pathogenies changed with different detection assays.Conclusions The multiplex realtime RT-PCR was proved to be more sensitive than the tradional RT-PCR in the detection of HFMD throat swabs,especially for severe cases,which can explain the composition of pathogen realistically." @default.
- W2392792600 created "2016-06-24" @default.
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- W2392792600 date "2011-01-01" @default.
- W2392792600 modified "2023-09-23" @default.
- W2392792600 title "Study on the Application of Multiplex Realtime Reverse Transcription-Polymerase Chain Reaction(RT-PCR)and Traditional RT-PCR in the Surveillance of Hand,Foot and Mouth Disease" @default.
- W2392792600 hasPublicationYear "2011" @default.
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