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- W2392804546 abstract "Objective To study the effects of sulfur mustard (SM) on lymphocytes and find sensitive parameter for diagnosis at early stage and provide some basis for treatment. Methods The DNA injury was determined with single cell gel electrophoresis, the contents of NO and NOS were measured by NO and NOS kits respectively and apoptosis of lymphocytes was determined by flow cytometry and gelose electrophoresis. Results DNA was obviously damaged after 4h, (DNA migration and percentage of DNA injured lymphocytes were significantly increased) and most prominent at 72 h, began to decrease at 5d. NO and NOS concentration were remarkably increased after 4 h, reached peak at 1 d and recovered since 3 d. Apoptosis of lymphocytes was significantly increased at 4 h, but was not found at 1 d and 3d. DNA LADDER and DNA MADDER were observed. Conclusion (1)SCG is a sensitive, simple and economic method in evaluating the cytotoxicity of SM in toxication and the degree of intoxication, and with good prospects in early diagnasis; (2)The cytotoxicity caused by the elevation of NO induced by the NOS activity after intoxication might be one of the mechanisms of SM intoxication; (3)Apoptosis participates in the process of SM intoxication also might be one of the mechanisms of SM intoxication." @default.
- W2392804546 created "2016-06-24" @default.
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- W2392804546 date "2001-01-01" @default.
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- W2392804546 title "Study on the effects of sulfur mustard intoxication on lymphocytes in rats" @default.
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