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- W2392804568 abstract "By means of Langendorff method,isolated mouse heart was perfusedwith Krebs-Hensleit (K-H) buffer solution.After 10 min equilibrium,the heartunderwent global ischemia of three different duration followed by 10 min reperfu-sion.During the first 5 min of reperfusion,coronary effluents were collected in ac-cordance with given sequence,in order to analyze Lactate Dehydrogenase(LDH)activity U/L as an index of cellular damage induced by ischemic-reperfusion.Is-chemia,classified into three groups (5min,10min,and 15min),caused a biphasicLDH release.The first one,which represented mainly ischemic injury,appeared inthe first 15s of reperfusion,and had higher value than that of control.The activi-ties of LDH release were(55.4±3.8),(86.8±5.4),and (96.2±6.8)U/L,forthe above 3 groups,respectively.The second peak appeared in 180 to 240 s ofreperfusion represented mainly reperfusion injury,and was lower than that of thefirst peak,but higher than control value.Arrhythmias,e.g.,premature ventricu-lar contraction (PVC) and ventricular tachycardia (VT) were observed during 5min reperfusion period.The numbers of PVC were 187±17,217±10,and 244±14,whereas the duration of VT was (10±2) s,(22±3)s,and(24±2)s,forabove 3 groups,respectively.Normal sinus rhythm time (NSRT) during earlyreperfusion period of the above 3 groups were measured to (214±SD29),(196± SD31) and (183±SD24) s,respectively.In this study and experimental ischemia-reperfusion model indicated by LDH release has been the first established mouseheart at our laboratory.This model had provided convenient approach and new pos-sibilities for further investigation and protection of cardiac ischemia-reperfusion in-jury." @default.
- W2392804568 created "2016-06-24" @default.
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- W2392804568 date "1996-01-01" @default.
- W2392804568 modified "2023-09-25" @default.
- W2392804568 title "The Patterns of Lactate Dehydrogenase Release of Isolated Mouse Heart in the Early Ischemia Reperfusion Period" @default.
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