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- W2392853399 abstract "OBJECTIVE: To eximine the effect of rapamycin combined with cisplatin on the inhibition of Hela cells in vitro and the expression of HIF-1α and VEGF.METHODS:Cervical carcinoma Hela cell were treated by rapamycin,cisplatin,rapamycin and cisplatin respectively.The inhibitory effects of chemotherapy on the proliferation of Hela cell were measured with MTT assay,and the combined effect of rapamycin and cisplatin were evaluated using King's formula.The expressions of mRNA and the protein of the HIF-1α and VEGF were detected using transcription-polymerase chain reaction(RT-PCR)and Western Blot.RESULTS: Both rapamycin and cisplatin showed inhibitory effect on proliferation of Hela cells and with dose-dependence in two concentrations of rapamycin(10 and 20 nmol/mL) and cisplatin(0.25 and 0.5 mg/mL) respectively.The synergistic therapeutic index qvalues were both more than 1.15,which showed synergistic effect.The expressions mRNA of HIF-1 α and VEGF of each group respectively was rapamycin combination with cisplatin(0.242±0.048,0.498±0.093),rapamycin(0.428±0.068,0.651±0.112),cisplatin(0.357±0.051,0.623±0.125).The expressions of mRNA of HIF-1α and VEGF were significantly downregulated in the combined group(P0.05);The expression of protein of HIF-1 α and VEGF mRNA of each group respectively was Rapamycin and cisplatin combination(0.514±0.092,0.409±0.082),rapamycin(0.625±0.132,0.650±0.114),cisplatin(0.635±0.120,0.623±0.102).The expressions of protein of HIF-1α and VEGF were significantly downregulated in the combined group(P0.05).CONCLUSION: Both rapamycin alone or combined use of rapamycin and cisplatin showed inhibitory effect on the proliferation of Hela cells,the combined use of rapamycin and the cisplatin showed synergistic effects in the chemotherapy of Hela cell,The expressions of mRNA and protein HIF-1α and VEGF were significantly downregulated by the combined group." @default.
- W2392853399 created "2016-06-24" @default.
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- W2392853399 date "2013-01-01" @default.
- W2392853399 modified "2023-09-28" @default.
- W2392853399 title "Effect of rapamycin combined with cisplatin on the growth of Hela cells in vitro and the expression of HIF-1α and VEGF" @default.
- W2392853399 hasPublicationYear "2013" @default.
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