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- W2392853818 abstract "Objective To establish the serum-free suspension culture of CHO-K1-SFS cell line and determine its biological characteristics. Methods Adherent CHO-K1 cells were cultured by gradually decreasing serum concentration,and the obtained CHO-K1 cells in low serum medium were inoculated at a density of 3 × 105cells / ml to 150 ml Erlenmeyer flask containing serum-free SFM4CHO medium and cultured in shaker. The growth curve of the obtained CHO-K1-SFS cells was plotted,the microbial contamination was tested,while the metabolism was analyzed,and the exogenous gene expression was determined. Results The obtained CHO-K1-SFS cell line was suitable for serum-free suspension culture,of which the maximum density reached 3. 8 × 106cells / ml when inoculated at an initial density of 3 × 105cells / ml,while the mean doubling time was 29. 7 h. No contamination with bacteria,fungi,viruses or mycoplasma was observed. The utilization rate of glucose was high,and a quantity of lactic acid was produced. Exogenous gene was effectively expressed. Conclusion The serum-free suspension culture of CHO-K1-SFS cell line was successfully established,which laid a foundation of high expression of exogenous gene." @default.
- W2392853818 created "2016-06-24" @default.
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- W2392853818 date "2014-01-01" @default.
- W2392853818 modified "2023-10-04" @default.
- W2392853818 title "Establishment and biological characteristics of serum-free suspension culture of CHO-K1-SFS cell line" @default.
- W2392853818 hasPublicationYear "2014" @default.
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