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- W2392867306 abstract "Objective To establish a convenient and feasible HPLC method for determination of cyclosporine A level in blood.Methods The blood samples was separated with column C18(5 μm×150 mm×4.6 mm)after an improved extraction.The mobile phase was a mixture of methanol,water,acetonitrile and isopropanol with the ratio of 25∶57∶18∶1.5.and the flow rate was 1.0 mL/min.The column temperature was maintained at 70 ℃ and the wave length of UV for detection was 208 nm.Results A good line relationship was obtained within the blood CsA level range from 50 ng/mL to 2 000 ng/mL(r=0.998 0).The minimal level of of CsA that could be detected was 50 ng/mL.The ABSolute recovery of CsA was 92.1%~95.7% and relative recovery of CsA was 94.3%~99.8%.Conclusion The method is accurate,simple,sensitive and suitable for therapeutic monitoring of CsA after transplantation." @default.
- W2392867306 created "2016-06-24" @default.
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- W2392867306 date "2009-01-01" @default.
- W2392867306 modified "2023-09-23" @default.
- W2392867306 title "Determination of Blood Cyclosporine A Level with HPLC" @default.
- W2392867306 hasPublicationYear "2009" @default.
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