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- W2392871308 abstract "ObjectiveTo provide high quality soluble leucine-rich repeats and immunoglobulin-like domains 1(sLRIG1) protein for further research on the treatment of malignant tumors.Methods Sf9 insect cells were infected with the recombinant sLRIG1 Baculovirus encoding the extra-cellular domain of human LRIG1(gene No.AF381545).The supernatant of the culture medium was collected and the recombinant sLRIG1 was purified by the Ni Sepharose Fast Flow Column and the DEAE Sepharose Fast Flow Column.The obtained protein was identified by SDS-PAGE technique and Western blotting.Results The sLRIG1 protein was successfully expressed and its expression reached peak at 72 hours after infection when the multiplicity of infection was 5.The purity was over 95%.Conclusion The sLRIG1 protein was successfully preparated via Baculovirus expression vector system.The purified protein had a high quality and was suitable for the further research on the treatment of malignant tumors." @default.
- W2392871308 created "2016-06-24" @default.
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- W2392871308 date "2012-01-01" @default.
- W2392871308 modified "2023-09-23" @default.
- W2392871308 title "Preparation of soluble leucine-rich repeats and immunoglobulin-like domains 1 protein via the Baculovirus expression vector system and its identification" @default.
- W2392871308 hasPublicationYear "2012" @default.
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