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- W2392901762 abstract "Hepatitis B surface antigen(HBsAg) carrying preS sequences could be an ideal candidate for a new hepatitis B virus(HBV) vaccine with higher efficacy. Here we report the success in achieving efficient and stable expression of hepatitis B virus S antigen and preS1 epitope fusion protein(S/preS1) in CHO cells. The HMRCHEF53u/Neo-S/preS1 expression vector carrying S/preS1 gene was constructed and transfected into CHO-S cells. A stable and high-expression CHO cell line, named 10G6, was selected by ELISA and limiting dilution analysis. Western blotting analysis showed S/preS1 expressed from 10G6 cells possessed both S and preS1 antigenicity. 10G6 cells displayed characters of favorable growth and stable S/preS1 expression in repeated batch cultures as evaluated by viable cell density, viability and S/preS1 concentration. And cultivation of 10G6 cells in fed-batch mode resulted in S/preS1 production at 17–20 mg/L with viable cell density at 7×106–10×106 cells/mL." @default.
- W2392901762 created "2016-06-24" @default.
- W2392901762 creator A5058997143 @default.
- W2392901762 date "2013-01-01" @default.
- W2392901762 modified "2023-09-23" @default.
- W2392901762 title "Stable and efficient expression of hepatitis B virus S antigen and preS1 epitope fusion protein(S/preS1) in CHO cells" @default.
- W2392901762 hasPublicationYear "2013" @default.
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