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- W2392904248 abstract "To establish ear marginal tissue fibroblast line of Datong horse from Qinghai for preserving the national important genetic resource in cell level,the ear marginal tissues of Datong horse were collected to prepare primary cells by tissue pieces method.The primary cells were purified and preserved with liquid nitrogen after being passaged to the thrid generation by differential digestion and differential adhesion,then the observations on morphology,dynamic growth,microbial contamination,analysis of karyotype,and the expression of fluorescent protein plasmid transfection were carried out.The results showed that the cell line was fibroblast-like and grew well.The maximum concentration for proliferation was 4.27×105/mL,and the population doubling time(PDT) of cells was 31.02 h;the tests for bacteria,fungi,virus and mycoplasma were all negative;the chromosome number was 64,and the diploid rate was 80%,which was dominant.It indicates that the red fluorescent protein plasmid can be replicated and expressed in the cell." @default.
- W2392904248 created "2016-06-24" @default.
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- W2392904248 date "2012-01-01" @default.
- W2392904248 modified "2023-10-03" @default.
- W2392904248 title "Establishment of ear marginal tissue fibroblast cell line and its biological characteristics in Datong horse from Qinghai" @default.
- W2392904248 hasPublicationYear "2012" @default.
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