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- W2392904673 abstract "Nested Primers based on special insertion sequence IS6110 of M.tuberculosis were designed,which were used to amplify M.tuberculosis genome DNA. This study developed the nested-PCR diagnosis method for detecting M.tuberculosis,which was verified with the specificity and sensitivity.244 bp fragment was obtained by amplifing the M.tuberculosis with the nested-PCR diagnosis method,Staphycococcus aureus,streptococcus,E.coli,Salmonella,Corynebacterium,Pseudomonas aeruginosa,Listeria monocytogenes,Brucella,et al were detected.The sensitivity of the nested-PCR diagnosis method is 4 copies/reaction by detecting the ladder concentration of simulation positive sputum samples.Forty one clinical samples of different places were detected,and the positive rate was 60.98%." @default.
- W2392904673 created "2016-06-24" @default.
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- W2392904673 date "2008-01-01" @default.
- W2392904673 modified "2023-09-28" @default.
- W2392904673 title "Preliminary Assessment of Nested-PCR Diagnostic Technique for M.tuberculosis" @default.
- W2392904673 hasPublicationYear "2008" @default.
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