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- W2392958466 abstract "Objective To combine lipid microbubbles with cationic nanoliposomes by biotin-avidin system as a type of gene HGF vector,in order to investigate the transfection and the cytoactive of hepatic stellate cell(HSC-T6).Methods HSC-T6was randomly divided into LMB group(given lipid microbubble and plasmid),LMB-CNLP group(given loadedgene combination of lipid microbubbles with cationic nanoliposomes) and contrast group(given a dose of plasmid).Expression of the gene HGF was observed on fluorescent microscopy and quantified with FACS analysis.ELISA was used to detect the expression of HGF.The viability of HSC-T6was measured with FACS analysis.Results Transfection efficiency in LMB-CNLP group was 41.29%,higher than that in the other two groups.The HSC-T6in LMB-CNLP group expressed HGF protein persistently.With HGF protein increased,more HSC-T6apoptosis was observed.Conclusion Combination of lipid microbubbles with cationic nanoliposomes can enhance the efficiency of gene HGF transfection in HSC-T6and promote cell apoptosis of HSC-T6,offering a experimental basis for the treatment of liver fibrosis." @default.
- W2392958466 created "2016-06-24" @default.
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- W2392958466 date "2012-01-01" @default.
- W2392958466 modified "2023-09-25" @default.
- W2392958466 title "In vitro combination of lipid microbubbles and cationic nanoliposomes in enhancing HGF gene transfection" @default.
- W2392958466 hasPublicationYear "2012" @default.
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