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- W2392981764 abstract "Objective To evaluate disk diffusion method for determining susceptibilities of Candida spp. . Methods The susceptibility of 136 strains of Candida spp. isolated from clinical specimens were tested to fluconazole by disk diffusion method (DDM) using the NCCLS document M44-P guideline. Disk test results were compared with results obtained by NCCLS M27-A2 broth microdilution method (BMM). Results In vitro susceptibility testing by DDM, Overall, 86% of Candida spp. were susceptible to fluconazole, 6. 6% were susceptible-dose dependent and 7. 4% were resistant. More than 90. 0% Candida albicans, Candida tropicalis, Candida parapsilosis and Candida guilliermonddi were susceptible to fluconazole. Candida krusei and Candida glabrata were lower susceptible, 0. 0% and 25% , respectively. The overall categorical agreement between disk diffusion test results and reference broth microdilution test results was 90.4% (123/136). Of the 123 isolates in all agreement, 113 were susceptible, 4 were susceptible-dose dependent, 6 were resistant. 2 isolates were resistant by BMM were identified as susceptible-dose dependent by DDM. Of the 8 isolates were susceptible-dose dependent by BMM, 4 isolates were susceptible and 4 isolates were resistant by DDM. 3 isolates were susceptible by BMM were identified as susceptible-dose dependent by DDM. It weren't found that the sensitive (or resistance) strains detected by BMM. However, the resistance (or sensitive) strains detected by DMM. The categorical agreement between results obtained for susceptible, susceptible-dose dependent and resistant by DMM and results obtained by BMM was 97.4% , 33. % and 75.0% , respectively. Conclusion The disk diffusion method is confirmed as a reliable approach to the detection of susceptibility of Candida spp. In vitro to fluconazole for application in routine laboratory." @default.
- W2392981764 created "2016-06-24" @default.
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- W2392981764 date "2005-01-01" @default.
- W2392981764 modified "2023-09-26" @default.
- W2392981764 title "Evaluation of disk diffusion method for determining susceptibilities of Candida spp. to fluconazole" @default.
- W2392981764 hasPublicationYear "2005" @default.
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