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- W2392990971 abstract "A novel diagnostic method was established to detect the presence of Mycoplasma suis by using the semi-nested PCR,in which the proper primers for PCR were designed by Oligo 6.0 and Primer 5.0 softwares and the cloned genes as demonstrated by analysis with enzyme digestion and the semi-nested PCR were proved to be the specific genes for M suis.Meanwhile,the sequence of clone M.suis was compared with that of M.suis published in GenBank(AJ504999).It was found that the sequence homology the clone M.suis with AJ504999 was 100%.As demonstrated by the specificity testing,the PCR primers designed in the present study could not be amplified to form bands from the genomic DNA of Toxoplasma gondii,Steptococcus species,E.coli,Mycoplasma hyopneumoniae of swine and pseudorabies virus.The lowest value of sensitivity of the semi-nested PCR as proved by the sensitivity testing was 0.12 fg DNA of the standard template.Compared with the conventional PCR,semi-nested PCR appeared to be more sensitive and more practicable,thus providing a reliable method to detect M.suis." @default.
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- W2392990971 date "2008-01-01" @default.
- W2392990971 modified "2023-09-24" @default.
- W2392990971 title "A diagnostic method to detect Mycoplasma suis using the semi-nested PCR" @default.
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