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- W2392993923 abstract "Objective: To elucidate the role of bone marrow stromal cells in cooperation with exogenous cytokines in hematopoiesis. Methods: Fetal bone marrow stromal cells (FBMSC) was combined with cytokines including SCF,IL 3,IL 6,GM CSF in a 5 day liquid culture system of adult bone marrow mononuclear cells, then we cultured bone marrow derived CD34 + enriched cells with FBMSC+SCF+IL 3+IL 6+G CSF+EPO for 2 weeks. Results:FBMSC were in good cooperation with above mentioned exogenous cytokines. When CD34 + enriched cells from adult bone marrow were cultured with combinations of FBMSC, SCF, IL 3, IL 6, G CSF and EPO, total nucleated cells, CFU GM, BFU E and CD34 + cells were increased by 119.6±30.9, 54.6±17.4, 25.2±4.4, 11.1±4.2 folds, respectively. Conclusion:FBMSC in cooperation with exogenous cytokines support the in vitro expansion of human hematopoietic progenitor cells efficiently." @default.
- W2392993923 created "2016-06-24" @default.
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- W2392993923 date "2001-01-01" @default.
- W2392993923 modified "2023-09-22" @default.
- W2392993923 title "Fetal bone marrow stromal cells support the in vitro expansion of human hematopoietic bone marrow progenitor cells: requirement for cell-to-cell interactions and colony-stimulating factors" @default.
- W2392993923 hasPublicationYear "2001" @default.
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