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- W2392995017 abstract "To establish the optimal system for extracting total RNA from leaves of Arabidopsis thaliana,the extraction effects of three methods,which including modified CTAB method,modified hot-phenol method and Trizol method,were compared,meanwhile,the expressions of heat stress related miRNAs were detected by using semi-quantitative RT-PCR technology.The results showed that using Trizol method can extract high quality total RNA of Arabidopsis thaliana,and its extracting effect was better than that of modified CTAB method and modified hot-phenol method;the expressions of the four screened miRNAs were induced by heat stress,but the expressions of each miRNA,which were treated at 38℃ for different time,were different,and with the time passed by,the expression level of each miRNA all was enhanced significantly." @default.
- W2392995017 created "2016-06-24" @default.
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- W2392995017 date "2012-01-01" @default.
- W2392995017 modified "2023-09-25" @default.
- W2392995017 title "Differential Expression of Heat Stress Related microRNA in Arabidopsis thaliana" @default.
- W2392995017 hasPublicationYear "2012" @default.
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