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• W2393013693 abstract "Objective To construct the fusion gene of soluble human stem cell factor (SCF) and enhanced green fluorescent protein (EGFP), and study the expression of the fused SCF-EGFP in CHO-K1 cells. Methods The standard cloning technology was employed to construct the SCF-EGFP fusion gene. The fusion gene was transfected into CHO-K1 cells by electroporation. The expression of the fusion gene was determined by flow cytometry (FCM) and fluorescence spectrophotometer. Results The expression of the fusion gene was observed in transiently transfected CHO-K1 cells. The fusion protein was secreted into supernatant. These data suggested that the EGFP tag did not interfere with the natural assembly and the biological activity of SCF molecule. Conclusion The SCF-EGFP fusion gene was constructed successfully and the fusion protein has activity." @default.
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• W2393013693 date "2004-01-01" @default.
• W2393013693 modified "2023-09-28" @default.
• W2393013693 title "Construction and Expression of Recombinant Gene for Fusion of Human Stem Cell Factor and EGFP" @default.
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