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- W2393015005 abstract "In this study the R1 region of P97 gene of Mycoplasma hyopneumoniae (Mhp) strain 168 was amplified by PCR using a pair of insite-mutation primers. The PCR product was inserted into expression plasmid pET-32a(+) .The recombinant plasmid was transformed into competent cells of BL21(DE3) and it contained correct object fragment by sequencing. It was induced to express by 0.1 mmol/L isopropyl-β-D-thiogalactoside (IPTG) at 37 ℃ for 4 hours. The dose of the recombinant protein was nearly 23% of the total product. The expression product of interest and its biological activities were characterized with western blotting and ELISA analyses." @default.
- W2393015005 created "2016-06-24" @default.
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- W2393015005 date "2005-01-01" @default.
- W2393015005 modified "2023-09-25" @default.
- W2393015005 title "Cloning and Expression of R1 Region of P97 Gene in Mycoplasma hyopneumoniae" @default.
- W2393015005 hasPublicationYear "2005" @default.
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