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- W2393018492 abstract "Fatty Acid Elongase(FAE1),existing widely in plants,is a ketoacyl-CoA,which can catalyse the prolongation reaction of fatty acid cabon chain on endoplasmic reticulum.Design primers according to the known sequence of Brassica napus FAE1 gene are promoted from GenBank.The total DNA,which is taken as the template for PCR,is extracted from the leaves of Braccica napus.L.The fragment was amplified and then cloned into pMD18-,T vector,and the homology is 99.78 percent after sequence alignment on the NCBI.The sequencing results indicated that the fragment maintained significant similarity to the known FAE1 gene promoter in plants.Then the fragment was inserted into pBI221 vector.The reconstructed plasmid,pBI221-,FP-,LUC,which had the Brassica napus FAE1 promoter and the reporter gene LUC,was transferred into protoplasts of Arabidopsis by PEG.Transient expression showed that the expression of LUC decreased when ABA was added." @default.
- W2393018492 created "2016-06-24" @default.
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- W2393018492 date "2011-01-01" @default.
- W2393018492 modified "2023-09-28" @default.
- W2393018492 title "Cloning and Transient Expression Assay of FAE1 Promoter from Brassica napus" @default.
- W2393018492 hasPublicationYear "2011" @default.
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