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- W2393019854 abstract "Objective:To introduce a modified rat model of middle cerebral artery occlusion(MCAO).Method:40 Sprague-Dawly male rats were used to induce focal cerebral ischemia reperfusion according to Longa's method.Focal cerebral blood flow(CBF),neurological deficit,cresyl violet(CV) stain and pathology were evaluated in all rats.Result:The operation proceeded about 15min after anaesthesia.Rats after operation presented severe neurological deficits companied with rCBF decreases.There were significant increases of brain water contents in ischemic brain regions.CV staining showed infarct areas and histological examinations revealed remarkable astrocyte swelling and neuronal necrosis.Conclusion:Modified methods is superior to traditional one.The cerebral blood flow reduction to 15% or less of baseline,ligation of pterygopalatine artery(PPA)are key factors in methods for rat model of cerebral ischemia reperfusion.Polysine head-coated nylon thread is less damage to blood vessel.The modified model is reliable,with the advantage of shortening operation time,is perfect for studying cerebral ischemia." @default.
- W2393019854 created "2016-06-24" @default.
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- W2393019854 date "2010-01-01" @default.
- W2393019854 modified "2023-09-23" @default.
- W2393019854 title "Improvement of Methods for Rat Model of Focal Cerebral Ischemia Reperfusion" @default.
- W2393019854 hasPublicationYear "2010" @default.
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