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- W2393020238 abstract "In order to establish an efficient genetic transformation system with leaves of Dendranthema morfolium Tzvel.cv.Xiaojinhuang,several factors affected genetic transformation mediated by Agrobacterium were studied.The results showed that the highest transformation efficiency was obtained through the following transformation procedure: after preculture for 2 d,the explants were infected 3 min with 50 mg/L acetosyringone(AS) and OD600=0.5 Agrobacterium culture liquid,and then co-cultivated for 2 d.Resistant shoots from leaf disks were screened on MS+0.4 mg/L NAA +100 mg/L Cef medium supplemented with 10 mg/L Km.Results of PCR proved that the CBF1 gene was integrated into the genome of transgenic plants,and eighteen transgenic lines were obtained,the transformation rate was 36.7%." @default.
- W2393020238 created "2016-06-24" @default.
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- W2393020238 date "2009-01-01" @default.
- W2393020238 modified "2023-09-28" @default.
- W2393020238 title "Agrobacterium-mediated Transformation of Deranthema morifolium Tzvel.cv.Xiaojinhuang with Transcription Factor CBF1 Gene" @default.
- W2393020238 hasPublicationYear "2009" @default.
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