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- W2393023727 abstract "In order to investigate thoroughly the enzymatic properties of CCR which plays critical role in lignin biosynthesis,we analyzed the catalytic characteristics of CCR from perspective of products formation.Chemical method was used for synthesis of 4-hydroxycinnamaldehydes(p-coumaraldehyde,coniferaldehyde,caffeylaldehyde)which are the products of CCR enzymatic reaction.4-Hydroxycinnamaldehydes were then purified by HPLC and detected by HPLC-MSn.Results showed that the synthesized 4-hydroxycinnamaldehydes can be used as standards in research of CCR.We also established a new method to detect CCR activity using HPLC-MSn.The recombinant CCR protein was expressed efficiently and purified by affinity chromatography.The CCR activity assay of the enzymatic reactions with multiple substrates was performed using HPLC-MS system.The results showed that the method can effectively monitor the change in both substrate and product,which provides new insights for the studyof catalytic characteristics of key enzymes in lignin biosynthesis." @default.
- W2393023727 created "2016-06-24" @default.
- W2393023727 creator A5017448729 @default.
- W2393023727 date "2015-01-01" @default.
- W2393023727 modified "2023-09-26" @default.
- W2393023727 title "Research of 4-hydroxycinnamaldehydes-production of CCR enzyme reaction" @default.
- W2393023727 hasPublicationYear "2015" @default.
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