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- W2393034008 abstract "Objective To investigate the methods and feasibility to establish experimental model of toxic cardiomyocytes induced by doxorubicin(DOX).Methods The primarily cultured neonatal SD rat cardiomyocytes were exposed to different concentrations of DOX and analyzed at different post-treatment durations after having been cultured for 4d.The cardiomyocytes viability was determined by MTT assay.The beating rate of cardiomyocytes was detected by inverted microscope.The activity of lactic dehydrogenase(LDH) and aspartate aminotransferase(AST) in superior fluid were measured by colorimetry.The cardiomyocyte apoptosis was detected by Hoechst 33258 staining.Results DOX had injury effect on cardiomyocytes with different concentration.The survival rate decreased significantly when the concentration was above 1.0mg/L.Accompanied with the DOX concentration increased,the beating rate of cardiacmyocytes decreased,and the activity of LDH and AST increased,the apoptosis rate increased significantly,compared with the control group(P0.05).Meantime,the longer the time DOX contacted with cardiacmyocytes,the lower the survival rate was.Conclusion The damage on cardiomyocytes induced by DOX is dose-dependent and time-dependent.The toxic cardiomyocytes model can be established when primary cultured cardiomyocytes are exposed to 1.0mg/L doxorubicin for 4h." @default.
- W2393034008 created "2016-06-24" @default.
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- W2393034008 date "2009-01-01" @default.
- W2393034008 modified "2023-09-24" @default.
- W2393034008 title "Establishment of toxic cardiomyocytes model induced by doxorubicin in vitro" @default.
- W2393034008 hasPublicationYear "2009" @default.
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