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- W2393035917 abstract "Objective To establish fluorescence-labeled mouse tumor model using GFP transgenic mice and DsRed labeled tumor cells,and whole-body imaging system and fluorescence microscopy were used to develop the technique of observation for the tumor in the whole-body and cell level directly.Methods B16 melanoma cells were implanted subcutaneously in GFP mouse to copy GFP mouse tumor model.DsRed labeled B16 cells were cloned with G418 selection(B16-RFP).B16-RFP cells were implanted into the mice subcutaneously to established tumor mouse.The whole-body imaging and fluorescence imaging technique was used to monitor the growth of the implanted tumor.Results A GFP mouse tumor model and a GFP mouse bearing DsRed tumor mouse model were successfully established.Under whole-body imaging scope,the green fluorescence tissue from the host mice and the red in tumor exhibited a fused growth.Under the fluorescence microscopy,the blood vessels and lymphatic cells from host was also exhibited in the tumor tissues.Using this technique and the model,the treatment of Lentinan was directly assayed,which showed that Lentinan significantly increased lymphatic cells and decreased the blood vessels in the tumor tissues compared with saline control.Conclusion We established GFP mouse bearing DsRed tumor models,which could be used for observation the tumor growth and interaction between host cells and tumor cells directly by whole-body imaging system and fluorescence microscopy." @default.
- W2393035917 created "2016-06-24" @default.
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- W2393035917 date "2008-01-01" @default.
- W2393035917 modified "2023-09-26" @default.
- W2393035917 title "Research of Fluorescence-labeled Technique on Tumor Mouse Model" @default.
- W2393035917 hasPublicationYear "2008" @default.
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