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- W2393040493 abstract "In order to solve the phenomenon of chimera in vitro shoots translating directly into seeding,wheat immature embryo of five common varieties were used as the experimental material to study the effect of plant growth regulators on formation of multiple shoots from stem apex of immature embryo in wheat. The results showed that,addition of different concentration of TDZ impacted on germination rate of immature embryo in wheat: with the concentration of TDZ increased within the scope of the design gradient,the germination rate of Xinong183 was increased,while Qianjinzao,Xiaoyan22,Yangmai12 and Yangmai18 were first increased and then decreased. When the concentration of TDZ was4mg·L- 1,the germination rate of Qianjinzao was the best with 92. 2%. Added TDZ to germinate was to the benefit of inducting multiple shoots. Different hormone specie,concentration and combination had a significant impact on the differentiation of multiple shoots in the program of induction. Under the premise of the best hormone concentration and combination,the regeneration rate of multiple shoots induct with 3mg·L- 1TDZ and 0. 5mg·L- 1IBA was the highest,3mg·L- 1TDZ and 1mg·L- 16- BA combined was better,then followed by 3mg·L- 1TDZ,1mg·L- 16- BA and 2mg·L- 14- PU. The best method of formation on the multiple shoots from immature embryo in wheat was 4mg·L- 1TDZ to germinate,3mg·L- 1TDZ and 0. 5mg·L- 1IBA to induct. Differentiation efficiency of the five wheat materials was different,Yangmai18 was the highest,then followed by Yangmai12,Qianjinzao,Xinong183 and Xiaoyan22. This result could provide some references to set up efficient induction system of multiple shoots from immature embryo in wheat and select better receptor of genetic transformation." @default.
- W2393040493 created "2016-06-24" @default.
- W2393040493 creator A5087187152 @default.
- W2393040493 date "2015-01-01" @default.
- W2393040493 modified "2023-09-25" @default.
- W2393040493 title "Effects of Plant Growth Regulators on Induction of Multiple Shoots From Stem Apex of Immature Embryo in Wheat" @default.
- W2393040493 hasPublicationYear "2015" @default.
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