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- W2393044973 abstract "The expression vector containing cDNA encoding a lysine-rich protein and bar gene was constructed. The mature embryonic calluses of Longtefu B and the primary embryo of Zhongyouzao 5 were transformed with expression vector by microprojectile bombardment. And sixteen fertile regenerated plants were obtained. Genomic PCR and Southern blotting analysis showed that the foreign genes were integrated into the rice genome. Resistance analysis showed that some T1 plants were resistant to herbicide. The analysis of protein and lysine contents revealed that in Zhongyouzao 5 transgenic seeds, the protein content (12.07%~16.20%) increased 35.18% as well as the lysine content (0.52%~0.67%) increased 45.09%, and in Longtefu B transgenic seeds the protein content (11.92%~12.87%) increased 27.69% as well as the lysine content (0.51%~0.57%) increased 16.15%." @default.
- W2393044973 created "2016-06-24" @default.
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- W2393044973 date "2004-01-01" @default.
- W2393044973 modified "2023-09-23" @default.
- W2393044973 title "Transfer of High Lysine-rich Gene and bar Gene into Rice by Microprojectile Bombardment and Detection of Their Transgenic Plants" @default.
- W2393044973 hasPublicationYear "2004" @default.
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