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- W2393048135 abstract "【Objective】To evaluate the yield of the CS-3000 Plus blood cell separator for collection of peripheral blood stem cells(PBSC) from ABO and Rh incompatible allogeneic donors and the feasibility of PBSC component infusion to the recipients without removal of erythrocytes or plasma.【Methods】The CS-3000 Plus blood cell separator was utilized to collect PBSC component from 14 allogeneic donors.Of all the donors,5 were ABO major incompatible,6 were minor incompatible,2 were both major and minor incompatible and the other 1 was Rh major incompatible to corresponding recipients.The amount of PBSC component was harvested,and the parameter was modified according to the hematocrit and mononuclear cells(MNC)of donors.The nucleated cell count,percentage of MNC,number of CD34+ cell and percentage of viable cell(trypan blue excluding rate) in the component were detected before infusion.At the time of infusion,the recipients were protected by measures including cortisone,basification,diuresis,etc.【Results】By twice-thrice performings on these 14 donors enough PBSCs were collected for transplantation.Altogether 30 apheresises were performed,the mean yield of nucleated cells was 5.65×1010,in which 96% to 98% were MNC.The harvested number of CD34+ cell was 6.52×106/kg.All the trypan blue exclusion rate was 100%.In ABO and Rh major incompatible or both ABO major and minor incompatible volumes of production were 50ml,there were 3~7ml packed erythrocytes;in ABO minor incompatible component,there were 15~25ml of plasma.These components were infused into the recipients without removal of erythrocytes or plasma and no haemolytic reaction was observed in any recipient,and their hematopoietic functions soon recovered.【Conclusion】The enough PBSC can be acquired by using blood cell separator CS3000 Plus,with the modified separation parameters,and the erythrocytes are reduced.The collected PBSC component can be safely infused into the blood group incompatible recipients without removal of erythrocytes or plasma." @default.
- W2393048135 created "2016-06-24" @default.
- W2393048135 creator A5029721216 @default.
- W2393048135 date "2007-01-01" @default.
- W2393048135 modified "2023-09-25" @default.
- W2393048135 title "Clinical Study of Collecting Peripheral Blood Stem Cells from Blood Group Incompatible Allogeneic Donors by Using CS-3000 Plus Blood Cell Separator" @default.
- W2393048135 hasPublicationYear "2007" @default.
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