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- W2393049133 abstract "Objective: To express and purify non-tagged Yersinia pestis LcrV antigen mutant in Escherichia coli and investigate its immunogenicity.Methods: Bases encoding Cys273 of LcrⅤ were deletion mutated by using fusion PCR techniques.This LcrV mutant gene was cloned into expression vector pET-32a(+) and then transformed into E.coli BL21(DE3).The mutant protein Vm was induced by IPTG,purified with chromatographic column,identified by Western blotting,and used to immunize BALB/c mice.Serum antibody was detected by ELISA.Results: Vm was expressed in soluble form at high levels.After purified with three steps,the purity was more than 95%.Anti-Ⅴ antibody could specifically react with Vm by Western blotting.BALB/c mice vaccinated with Vm induced a strong and specific antibody.Conclusion: The non-tagged mutant protein Vm with strong immunogenicity was obtained,which could be helpful to study the structure and function of LcrⅤ." @default.
- W2393049133 created "2016-06-24" @default.
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- W2393049133 date "2012-01-01" @default.
- W2393049133 modified "2023-09-27" @default.
- W2393049133 title "Expression and Purification of Non-Tagged Yersinia pestis LcrV Antigen Mutant in Escherichia coli and its Immunogenicity in BALB/c Mice" @default.
- W2393049133 hasPublicationYear "2012" @default.
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