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- W2393055663 abstract "The key factors on high-level fusion protein HSA-CP production in recombinant Pichia pastoris were investigated in shake flask.It showed that with 10 g/L of methanol induced for 72 h,the yield of the target protein was high.By studying the yield of the target protein under different cultivation conditions in 7 L fermentor,the results were obtained as follows:firstly kept at pH 5.0 and 30% dissolved oxygen during the incubation period,then at pH 6.0 and 15% dissolved oxygen during induction period,with initial glycerol concentration of 10 g/L at 30 ℃ for 72 h,dry cell mass.Finally,the maximum concentration of fusion protein reached 56.43 g/L and HSA-CP was 368.45 mg/L.The productivity reached 3.920 mg/(L·h),the specific productivity of the target protein was 5.12 mg/(L·h)." @default.
- W2393055663 created "2016-06-24" @default.
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- W2393055663 date "2009-01-01" @default.
- W2393055663 modified "2023-09-23" @default.
- W2393055663 title "Fermentation conditions of optimization of HSA-CP fusion protein expressed in Pichia pastoris" @default.
- W2393055663 hasPublicationYear "2009" @default.
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