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- W2393057194 abstract "Objective To evaluate on measuring vaginal sialidase activity to identify bacterial vaginosis(BV) by using 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid(X-Neu5Ac) as the substrate.Methods The study population comprised 45 of them with clinical signs of BV(BV+) and 30 of them without BV(BV-).Bacterial vaginosis was diagnosed by clinical criteria.The vaginal fluids from suspected patients were evaluated by sialidase levels and collected for quantitative determination,including Lactobacillus spp,Bacteroides spp,Escherichia coli,Staphylococcus spp,Streptococcus spp,Gardnerella vaginalis.Sialidase activity using X-Neu5Ac as the substrate,specific activity was expressed as nanomoles of methoxyphenol produced.The method of measuring vaginal sialidase activity was compared to the standard method for diagnosing BV(Amsel criteria).Results The sensitivity and specificity of the test for prediction of BV were 88.9% and 90%,respectively,with no significant differences(P0.05) from those by Amsel criteria.Positive and negative predictive values were 93% and 84.3%,respectively.The mean Gardnerella vaginalis count in the sialidase-positive group was 6.95 log colony-forming units(CFU)/g,which was significantly higher(P0.01) than that(2.05 log CFU/g) in the sialidase-negative group.The mean count of hydrogen peroxide-producing Lactobacilli(LB+) species was significantly higher(P0.05) in the sialidase-negative group than in the positive counterpart(4.26 vs 8.66).In contrast,there was no difference in the mean total aerobe count between the two groups,including Staphylococcus spp,Enterococcus spp and Escherichia coli.Conclusions The vaginal sialidase activity for identifing bacterial vaginosis(BV) by using X-Neu5Ac as the substrate was a useful diagnostic tool of BV.Elevation of vaginal sialidase activity,an increase in the anaerobe count,and decreases in LB+ count could be good predictors of the deterioration of bacterial vaginosis." @default.
- W2393057194 created "2016-06-24" @default.
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- W2393057194 date "2006-01-01" @default.
- W2393057194 modified "2023-09-26" @default.
- W2393057194 title "Diagnosis of bacterial vaginosis by detection of sialidase activity using 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid as the substrate" @default.
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