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- W2393060076 abstract "[Purpose] To investigate the effect of VEGF-C antisense oligodeoxynucleotide(ASODN) on the invasion and metastasis of human ovarian cancer cell line SKOV3. [Methods] The ASODN sequence targeting the coding region of VEGF-C was synthesized and transfected into ovarian cancer cells by liposomes.The adhesion of SKOV3 cells to artifical basement membrane Matrigel was measured by MTT assay. The invasion was evaluated by transwell chambers attached with polycarbonate filters and reconstituted basement membrane(Matrigel). The expression of VEGF-C, MMP-2 and E-cadherin protein was measured by Western blot assay. [Result] The result of adhesion experiment demonstrated that VEGF-C ASODN markedly suppressed the adhesion ability of SKOV3 cells to Matrigel at 30min, 60min, 90min and 120min compared with the control group and the lipofectin (LIP) group and the sense oligodeoxynucleotide (SODN) group (P0.01). The result of invasion experiment demonstrated the invasive cell number in VEGF-C ASODN group (62.5±8.7) decreased significantly than that in the control group, the lipofectin group and SODN group respectively (127.2±13.6, 125.8±14.1 and 119.3±11.2, P0.01). The result of Western blot showed the expression of VEGF-C and MMP-2 protein in VEGF-C ASODN group was remarkably down-regulated and E-cadherin protein up-regulated compared with the control group, the lipofectin group and SODN group (P0.01). [Conclusion] VEGF-C ASODN can inhibit invasion and metastasis of SKOV3 cells remarkably, which might become an effective adjuvant therapy for ovarian cancer in the future." @default.
- W2393060076 created "2016-06-24" @default.
- W2393060076 creator A5028973264 @default.
- W2393060076 date "2008-01-01" @default.
- W2393060076 modified "2023-09-23" @default.
- W2393060076 title "Effect of VEGF-C Antisense Oligodeoxynucleotide (ASODN) on Invasion and Metastasis of Ovarian Cancer Cells SKOV3" @default.
- W2393060076 hasPublicationYear "2008" @default.
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