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- W2393065913 abstract "METHODS:Crude antigens from colorectal cancer tissue and crude butanol extraction(CBE) from colorectal cancer cell line HRT-18 were extracted with detergent Triton X-100 and 1-butanol,respectivelyBoth of them can be bound to the associated monoclonal antibody Hb3.Colorectal cancer-associated antigen CA-Hb3 was obtained after crude antigen and CBE were further purified through Hb3-Sepharose 4B affinity column.RESULTS:CA-Hb3 is a glycoprotein(about 50kD)which may be a homodimer contained two subunits with molecular weight of about 27kD and its antigenic determinant locates on carbohydrate chain was found by analysis with SDS-PAGE,Western-and Dot-blotCONCLUSION:It may be a good way to boil samples for different time for denaturation in the procedure fo SDS-PAGE for analysing subunits of glycoproteins." @default.
- W2393065913 created "2016-06-24" @default.
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- W2393065913 date "1998-01-01" @default.
- W2393065913 modified "2023-09-25" @default.
- W2393065913 title "Extraction affinity purification and biological characteristics of colorectal cancer-associated antigen CA-Hb3" @default.
- W2393065913 hasPublicationYear "1998" @default.
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