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- W2393067319 abstract "OBJECTIVE:To establish an HPLC method for the determination of methotrexate level in serum of children with acute lymphoblastic leukemia(ALL).METHODS:Blood sampling were performed at 24,44 and 68 h after the initiation of high-dose methotrexate infusion in ALL children and the serum samples were treated with perchloric acid for sedimentation and high speed centrifugation,then the supernatant was taken for analysis.Chromatographic separation was performed on Syne-rgi-4u Fusion-RP 80A analytical column with mobile phase consisted of phosphate buffer(pH6.8)-methanol (78∶22) at a flow rate of 0.8 mL·min-1.The UV detection wavelength was set at 306 nm and the column temperature was 30 ℃. RESULTS: The calibration curves of methotrexate were linear at a concentration range from 0.05 to 2.50 μmol·L-1(r=0.999 1) or from 2.50 to 100.0 μmol·L-1(r=0.999 8),with intra-day and inter-day RSD at (4.09±1.69)% and (4.21±1.27)% respectively.The average method recovery was(99.86±1.38) % and the average extraction recovery was(68.85±1.46)%,with a lowest limit of 0.05 μmol·L-1.At 24,44 and 68 h,the average serum methotrexate concentrations in the 20 ALL children were(38.2±10.7),(0.46±0.36) and (0.10±0.07)μmol·L-1,respectively.CONCLUSION:The determination of methotrexate concentration in serum is simple,accurate and reliable,and in which the sample size is small,thus this method is suitable for the blood drug concentration monitoring of methotrexate in the clinic." @default.
- W2393067319 created "2016-06-24" @default.
- W2393067319 creator A5028237815 @default.
- W2393067319 date "2008-01-01" @default.
- W2393067319 modified "2023-09-27" @default.
- W2393067319 title "Determination of Serum Concentration of Methotrexate in Children with Acute Lymphoblastic Leukemia by HPLC" @default.
- W2393067319 hasPublicationYear "2008" @default.
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