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- W2393070775 abstract "Objective:To construct eukaryotic expression vector for anti-human melanoma chimeric antibody,and express the antibody in eukaryotic cells.Methods:Variable regions of the murine antibody were cloned from the anti-human melanoma hybridoma cell line HB8459,HB8760,Mel3,and was inserted them into the chimeric antibody eukaryotic expression vector pMH-CA.The constructed vector was transfected into 293T,the chimeric antibody was expressed,as detected by RT-PCR,ELISA,and Western blot.Results:Eukaryotic expression vectors of the chimeric antibody against human melanoma were successfully constructed,and expressed in eukaryotic cells.The expression of chimeric gene was confirmed by RT-PCR,specificity of the chimeric antibody was proved by ELISA and Western blot.Conclusion:Anti-human melanoma chimeric antibody was expressed successfully." @default.
- W2393070775 created "2016-06-24" @default.
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- W2393070775 date "2005-01-01" @default.
- W2393070775 modified "2023-09-24" @default.
- W2393070775 title "Construction and expression of mouse-human chimeric anti-human melanoma antibody" @default.
- W2393070775 hasPublicationYear "2005" @default.
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