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- W2393077921 abstract "Objective: To investigate the expression of vascular endothelial growth factor(VEGF) in mouse melanoma cell B16-F10 induced by PMA and hypoxia so as to set up a stable in vitro model for RNA interference.Methods: Mouse VEGF immunoassay(ELISA kit) was utilized to determine the VEGF concentration secreted by B16-F10 cell.Confocal laser scanning microscopy was used for visualization of cellular uptake of siRNA loaded nanoparticles as well as cell morphology.Results: Stimulation of B16-F10 cells with 1 M PMA for 2 h,VEGF expression increased.Following hypoxia incubation for 48 h,the concentration of VEGF increased significantly 200%,ranging from 55 to 65 pg/mL/h in comparison with routine culture.Conclusions: Combining PMA stimulation and hypoxia culture,VEGF expression in B16-F10 cells was up-regulated and could keep a stable level in 48 h,which suggested that the induced B16-F10 cells were suitable for in vitro RNAi study.Furthermore,the silencing efficiency of VEGF by TKO/siRNA nanoparticles and chi-tosan/siRNA nanoparticles showed preliminary result of 40% with the established B16-F10 cell model." @default.
- W2393077921 created "2016-06-24" @default.
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- W2393077921 date "2012-01-01" @default.
- W2393077921 modified "2023-09-27" @default.
- W2393077921 title "Construction of VEGF Up-regulated Cells Induced by PMA and Hypoxia for RNAi" @default.
- W2393077921 hasPublicationYear "2012" @default.
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