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- W2393080927 abstract "The ribosomal internal transcribed spacer regions (ITS 1 and ITS 2) and mitochondrial gene fragments ( 16S rDNA and COI) of Crassostrea gigas were amplified via PCR, and the PCR products were ligated into T vectors, cloned and sequenced.The nucleotide sequences of 543,791,530 and 700 bp from ITS 1,ITS 2,partial 16S rRNA gene and partial COI gene were obtained, respectively. The contents of A, T, G and C were 23.57%, 20.07%, 29.47% and 26.89% in ITS 1, 27.43%, 19.22%, 27.05% and 26.30% in ITS 2, 29.25%, 29.25% 23.02% and 18.49% in 16S rDNA, and 22.71%, 39.43%, 20.43% and 17.43% in COI in turn. The primers of ITS 1 and ITS 2 proved to be very universal in a variety of mollusk species. The potential uses of these four sequences for species identification and relevant research of several closely related oyster species were discussed." @default.
- W2393080927 created "2016-06-24" @default.
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- W2393080927 date "2002-01-01" @default.
- W2393080927 modified "2023-09-25" @default.
- W2393080927 title "Sequencing of ribosomal internal transcribed spacer regions and mitochondrial gene fragments in Crassostrea gigas" @default.
- W2393080927 hasPublicationYear "2002" @default.
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