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- W2393128652 abstract "Using the transient expression of GUS gene, we optimized the physical and biological condition of bombardment for micro-cross section of fruit banana (Musa spp.). The results suggested that osmotic treatment influence expression obviously. Transient expression rates of control and osmotic treatment from 4 h before to 16 h after the bombardment were 3 .68% and 14. 11 %, respectively. Osmotic treatment caused 3. 86 folds increase of the transient expression. The GUS expression can be improved several folds by the preculture of explants. The best pressure and the best distance from micro- carrier to target cell were 7.584 MPa and 9 cm, respectively. Calcium ions and sperimidine were nec- essary to the transient expression. The effect of CaCl_2 or Ca (NO_3 )_2 at either pH 5. 0 or 10. 0 on GUS expression were also studied. CaCl_2 solution at pH 5. 0 in the mixture was superior to the others. Under the above optimal conditions, 48.72% of transient GUS expression rate could he obtained." @default.
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- W2393128652 date "2000-01-01" @default.
- W2393128652 modified "2023-09-28" @default.
- W2393128652 title "Optimization of the Biological and Physical Parameters of Bombardment - Mediated Transformation of Micro-Cross Section Explants of Banana" @default.
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