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- W2393140189 abstract "The insect cellvirus (AcNPV) system was used to express heterologous protein. The recombinant transfer vector pVL1393/hGH was reconstructed in which the human Growth Hormone (hGH) gene was inserted under the control of the polyhedron gene promoter. The Spodoptera frugiperda (Sf9) cells was cotransfected with the plasmid DNA containing hGH gene and wildtype AcNPV DNA. The hGH gene was transferred to the AcNPV genome DNA through homologous recombination, and the recombinant virus rAcVhGH was obtained by multiple plaque purification. The high level of production of hGH (40 μg/mL) in supernatant of the infected monolayer culture was determined by immunochemiluminescent assay." @default.
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- W2393140189 date "1998-01-01" @default.
- W2393140189 modified "2023-09-23" @default.
- W2393140189 title "Expression of the Human Growth Hormone Genein Insect Cells" @default.
- W2393140189 hasPublicationYear "1998" @default.
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