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- W2393182182 abstract "AIM:To investigate the physiological role of DBP in bile acid biosynthesis through determining the change of its expression and activity when bile acid biosynthesis increases.METHODS:To activate LXR and increase bile acid synthesis,C57BL/6J mice were fed T0901317 for 7 d and Wistar rats were fed a high-cholesterol diet for 2 wk.Fecal bile acid was measured by auto-biochemical-analysis apparatus.The expression of CYP7A1 and DBP mRNA was analyzed by RT-PCR.DBP protein was estimated by Western Blot.DBP activity was measured by spectrophotometry.RESULTS:Fecal bile acids(P0.05),the mRNA expression of CYP7A1(P0.05)and DBP(P0.01),and activity of DBP(P0.05)in liver of C57BL/6J mice treated with T0901317 and Wistar rats fed high-cholesterol diet were higher than that in the corresponding control groups.The liver protein level of DBP in mice treated with T0901317 also increased(P0.05).CONCLUSION:DBP is involved in the bile acid biosynthesis in vivo." @default.
- W2393182182 created "2016-06-24" @default.
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- W2393182182 date "2008-01-01" @default.
- W2393182182 modified "2023-09-23" @default.
- W2393182182 title "Correlation of bile acid synthesis increase with hepatic DBP mRNA level and activity" @default.
- W2393182182 hasPublicationYear "2008" @default.
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