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- W2393183764 abstract "Aim To establish a method of enhancing exogenous bone marrow derived dendritic cells (BM-DC) homing to draining lymph nodes by induction of local mast cell degranulation. Methods Compound 48/80 (c48/80) was injected into C57BL/6 scapular skin to induce local mast cell degranulation. BM-DC generated from bone marrow of syngenic mice were labeled with YG-Microspheres and injected into c48/80 or normal saline treated scapular skin. Cells derived from draining lymph nodes (DLN) were pooled together 48 h after BM-DC injection and stained with conjugated anti-CD11c. The efficiency of BM-DC homing to lymph nodes was analyzed by FCM. Results Mast cell degranulation was locally boosted by c48/80 injection and caused enhancement of the homing of exogenous BM-DC to DLN, with an increase of 67%+/-43%. The total cells in lymph nodes also increased significantly. The fold of increase was 55%+/-43%. Conclusion Exogenous BM-DC homing to DLN can be boosted by inducing local skin mast cell degranulation." @default.
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- W2393183764 date "2010-07-01" @default.
- W2393183764 modified "2023-09-26" @default.
- W2393183764 title "Mast cell degranulation enhances exogenous dendritic cells homing to draining lymph nodes" @default.
- W2393183764 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20619086" @default.
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