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- W2393187069 abstract "Objective: To establish a recombinant strain over-expressing catalytic region of glucosyltransferase(GTF-I,containing B-Cell epitope) from Streptococcus sobrinus 6715 for the study of anti GTF-I monoclonal antibody and the sub-unit of anti-caries vaccine. Methods: Expressing plasmid pGEX-gtf was constructed from the vector pGEX-4T-1 and the target PCR amplified product(1.1 kb) and then transformed into host strain Escherichia coli JM109. The recombinant strain harboring the pGEX-gtf plasmid was verified by PCR. The expression of the gene in the recombinant strain was also tested by SDS-PAGE before and after induction by isopropy-β-D-thiogalactosid(IPTG). Results: The strain expressed GST-GTF fusion protein.Conclusion: GTF-1 expressing plasmid can produce targeted protein in the prokaryote expressing system" @default.
- W2393187069 created "2016-06-24" @default.
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- W2393187069 date "2004-01-01" @default.
- W2393187069 modified "2023-09-25" @default.
- W2393187069 title "Cloning and expression of gene encoding catalytic region of glucosyltransferase from Streptococcus sobrinus 6715" @default.
- W2393187069 hasPublicationYear "2004" @default.
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