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- W2393194878 abstract "A clone which can restore the ability of bdhA mutant strains NGRPA2 and Rm11107 to utilize 3 hydroxybutyrate as sole carbon source (Hbu +) was screened out by complementation experiment from Bradyrhizobium japonicum USDA110 genomic library. It was confirmed by Bdh assay and Southern blot that this clone contains bdhA gene. The entire sequence of bdhA gene was sequenced and the sequence was deposited in GenBank at the accession number of AY077581. bdhA gene consists of 789 base pairs and encodes Bdh with 262 amino acid and MW 27.59 ku. Interposon ΩKm was inserted into the bdhA ORF at EcoRⅠsite and the bdhA mutant was constructed in B.japonicum by homologous recombination. Plant test result did not show obvious effects of mutation of bdhA gene on nodulation and nitrogen fixation." @default.
- W2393194878 created "2016-06-24" @default.
- W2393194878 creator A5014192163 @default.
- W2393194878 date "2003-01-01" @default.
- W2393194878 modified "2023-09-25" @default.
- W2393194878 title "Cloning, Sequence and Characteristics of 3-Hydroxybutyrate Dehydrogenase Encoding Gene (bdhA) in Bradyrhizobium japonicum USDA110 Strain" @default.
- W2393194878 hasPublicationYear "2003" @default.
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