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- W2393215170 abstract "Aim To investigate the role of calcium in tumor necrosis factor α(TNF-α)-induced cardiomyocyte hypertrophy.Methods The protein content was assayed with Lowry's method.The cardiomyocytes volumes were measured by computer photograph analysis system.The protein synthesis was assayed with -leucine incorporation method.i transient was measured by Till image system by cell-loading Fura-2/AM.Results 2-APB,a selective IP3R inhibitor,and/or ryanodine,a selective RyR inhibitor,significantly suppress the elevation of the amplitude and the rates of the spontaneous Ca2+ transients induced by TNF-α in cultured ventricular myocytes from the neonatal rat.Nifedipine,an L-type calcium channels antagonist,had little effect on it.The increase of protein content,3H-leucine incorporation and cell size induced by TNF-α were significantly suppressed by 2-APB,and/or ryanodine.Nifedipine had no effect on it.Conclusion TNF-α induced cardiac hypertrophy through increasing of intracellular Ca2+ in cultured ventricular myocytes from the neonatal rat.It was induced by both IP3R and RyR,not by L-type calcium channels." @default.
- W2393215170 created "2016-06-24" @default.
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- W2393215170 date "2013-01-01" @default.
- W2393215170 modified "2023-09-25" @default.
- W2393215170 title "The Role of Calcium in Cardiomyocyte Hypertrophy Induced by Tumor Necrosis Factor-α" @default.
- W2393215170 hasPublicationYear "2013" @default.
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