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- W2393218071 abstract "A in situ hybridization(ISH) method was used to detect low copy FMDV in infected cells,in order to illuminate FMDV multiplication site.In this study,FMDV RNA was located and detected in BHK-21 cells by ISH.Cells infected experimentally with O/Akesu/58(10~7TCID_(50)) at 2,4,6,8 and 10 hours post-inoculation.Oligonucleotide sequences were chosen from the highly conversed regions of 3D gene of FMDV RNA.Probes were labeled with digoxigenin-11-dUTP at the 3′-end.Hybrids were detected by enhanced sensitive ISH detection kit Ⅱ(AP).Results showed that positive signals were found in the cytoplasm of infected cells post-inoculation,lots of viruses gathered periphery nucleus.Intensive positive signals showed in the cytoplasm of infected cells at 6,8 and 10 hours post-inoculation.So,we suggested ISH combined with enhanced sensitive ISH detection kitⅡ(AP) is a sensitive method,which was a reliable method for detection and localization of low copy viruses in infected cells.Results indicated multiplication and duplication of FMDV were in the cytoplasm of infected cells." @default.
- W2393218071 created "2016-06-24" @default.
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- W2393218071 date "2005-01-01" @default.
- W2393218071 modified "2023-09-25" @default.
- W2393218071 title "The Localization and Detection of Foot-and-Mouth Disease Virus in BHK-21 Cells by in situ Hybridization" @default.
- W2393218071 hasPublicationYear "2005" @default.
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