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- W2393252914 abstract "Objective To explore the kinetic changes in calcium level in neuron and establish a method to measure the quantity variation of intracellular calcium. Methods Neuron were cultured and stimulated with NMDA.Fluorescence microscope, computer and high-resolution digital CCD were applied to measure the quantity variation of intracellular free calcium.Results The fluorescence image analysis by fluorescence microscopic digital imaging system showed that under the stimulation of NMDA the quantity of intracellular free calcium in neuron was changed rapidly, and the whole trace of variation had been recorded.Conclusions Fluorescence microscopic digital imaging system is applicable for detecting the kinetic changes in intracellular calcium level and is a good substitute for laser scanning confocus microscope as a measurement to detect the changes in intracellular calcium level." @default.
- W2393252914 created "2016-06-24" @default.
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- W2393252914 date "2005-01-01" @default.
- W2393252914 modified "2023-09-24" @default.
- W2393252914 title "A Method Measuring Quantity Variation of Intracellular Free Calcium in Neuron with Fluorescence Microscopic Digital Imaging System" @default.
- W2393252914 hasPublicationYear "2005" @default.
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